Proteoglycan electrophoresis on horizontal submerged polyacrylamide-agarose gels

A method of proteoglycan electrophoresis on submerged horizontal polyacrylamide-agarose gels is described. Several preparations of purified proteoglycans extracted from fetal and young baboon articular cartilage and from mandibular dog-fish cartilage were analyzed. Discrete bands corresponding to proteoglycan monomers of different size were obtained. The results were similar to those obtained using the more tedious electrophoretic separation on cylindrical gel rods.