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Freshly-mixed and setting calcium-silicate cements stimulate human dental pulp cells
Mariano S Pedano1, Xin Li2, Shuchen Li3
1KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT, Campus Sint-Raphaël, block A - box 7001, Kapucijnenvoer 7, 3000 Leuven, Belgium; University Hospitals Leuven (UZ Leuven), Dentistry, Campus Sint-Raphaël, block A - box 7001, Kapucijnenvoer 7, 3000 Leuven, Belgium.
Summary
Freshly-mixed hydraulic calcium-silicate cements (hCSCs) are non-cytotoxic to human dental pulp cells (HDPCs). These cements, including one with phosphopullulan (PPL), promote HDPC proliferation, migration, and differentiation.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cell Biology
Background:
- Hydraulic calcium-silicate cements (hCSCs) are widely used in dentistry.
- Understanding their biological effects on dental pulp cells is crucial for clinical applications.
- Newly developed hCSCs require thorough evaluation for biocompatibility and bioactivity.
Purpose of the Study:
- To assess the impact of eluates from freshly-mixed and setting hCSCs on human dental pulp cells (HDPCs).
- To investigate the specific effects of a novel hCSC containing phosphopullulan (PPL) on HDPCs.
- To evaluate cytotoxicity, proliferation, migration, and odontogenic differentiation of HDPCs exposed to hCSC eluates.
Main Methods:
- HDPCs, characterized as mesenchymal stem cells, were utilized.
- Eluates were collected from experimental hCSC with PPL, Nex-Cem MTA, and Biodentine after 24-hour incubation.
- Cytotoxicity, proliferation (XTT assay), migration (wound-healing assay), and odontogenic differentiation (RT-PCR) were assessed at various dilutions.
Main Results:
- Eluates from the three freshly-mixed and setting hCSCs demonstrated no cytotoxicity towards HDPCs.
- All tested hCSCs significantly stimulated HDPC proliferation.
- Enhanced migration and odontogenic differentiation capabilities were observed in HDPCs exposed to the hCSC eluates.
Conclusions:
- Testing freshly-mixed and setting hCSCs provides a relevant model for evaluating material effects on HDPCs.
- The experimental hCSC containing PPL is non-cytotoxic and exhibits positive biological effects on HDPCs.
- hCSCs show promising biocompatibility and bioactivity, supporting their use in dental pulp regeneration and repair.
Keywords:
Bioactive materialsBiocompatibilityCytotoxicityPhosphopullulanPortland cementPulpotomyTricalcium silicateMore Related Videos
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