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Updated: Feb 13, 2026

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
Published on: July 11, 2017
A Biologist-Friendly Method to Analyze Cross-Correlation Between Protrusion Dynamics and Membrane Recruitment of
Perrine Paul-Gilloteaux1,2,3, François Waharte1,2, Manish Kumar Singh1,4
1Institut Curie, Centre de Recherche, Paris Sciences et Lettres Research University, Paris, France.
Abstract:
During mesenchymal cell motility, various actin regulators are recruited to the leading edge with exquisite precision in time and space to generate protrusion and retraction cycles. We present here an automated method, named CorRecD (from Correlation Recruitment Dynamics), which quantifies cell edge dynamics, protein recruitment and analyze their cross-correlation. The Wave Regulatory Complex (WRC), a master driver of protrusions, is used as a case-of-study. This biologist-friendly method relies on free software tools and can be applied to any fluorescently tagged protein of interest.
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