Related Experiment Video

Updated: Feb 13, 2026

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

4.8K

MicroRNA 26a Expression in Peripheral Blood Mononuclear Cells and Correlation with Serum Interleukin-17 in

Fatma M Mahmoud1, Nadia M ElSheshtawy1, Wafaa K Zaki1

  • 1Department of Medical Microbiology & Immunology, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.

The Egyptian Journal of Immunology
|March 13, 2018
PubMed

Insights

This study found increased levels of microRNA 26a (miR26a) and Interleukin 17 (IL17) in patients with relapsing-remitting multiple sclerosis (MS). These biomarkers may play a role in MS pathogenesis.

Area of Science:

  • Neuroimmunology
  • Molecular Biology

Background:

  • Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease.
  • T helper 17 (Th17) cells are key players in MS pathogenesis.
  • MicroRNAs are increasingly recognized for their role in Th17 cell differentiation and potential therapeutic applications.

Purpose of the Study:

  • To investigate microRNA 26a (miR26a) expression in peripheral blood mononuclear cells of relapsing-remitting MS patients.
  • To compare miR26a levels with healthy controls.
  • To examine the association between miR26a levels and serum Interleukin 17 (IL17) in MS patients.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) was used to measure miR26a expression.
  • Enzyme-linked immunosorbent assay (ELISA) was employed to determine serum IL17 levels.
  • Forty relapsing-remitting MS patients and twenty healthy controls were included in the study.

Main Results:

  • A significant upregulation of miR26a and IL17 was observed in total MS patients and those in the remitting phase compared to controls (P < 0.001).
  • The relapsing MS group showed a significant increase in miR26a expression (P= 0.004) but not IL17 levels.
  • No significant correlation was found between miR26a expression and serum IL17 levels in MS patients (r= 0.08, P= 0.62).

Conclusions:

  • Both miR26a and IL17 are significantly elevated in relapsing-remitting MS patients.
  • These findings suggest that miR26a and IL17 are important biomarkers in the pathogenesis and development of MS.

More Related Videos

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data10:46

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data

Published on: December 9, 2015

11.1K
Comprehensive Autopsy Program for Individuals with Multiple Sclerosis09:41

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Published on: July 19, 2019

12.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.5K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

4.0K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K