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Updated: Feb 13, 2026
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
Published on: May 4, 2011
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A multifunctional material based on co-electrospinning for developing biosensors with optical oxygen transduction.
Teresa Ramon-Marquez1, Antonio L Medina-Castillo2, Naveen Nagiah2
1Department of Analytical Chemistry, University of Granada, Avd. Fuentenueva s/n, 18071, Granada, Spain.
Analytica Chimica Acta
|March 14, 2018
Summary
A novel co-electrospun material with coaxial nanofibers enables optical oxygen biosensors. This versatile platform effectively immobilizes enzymes for accurate uric acid detection in serum samples.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Development of advanced materials for biosensor applications is crucial.
- Optical oxygen transduction offers a sensitive detection method.
- Coaxial nanofibers provide a unique structure for material functionalization.
Purpose of the Study:
- To develop a multifunctional material for biosensors using co-electrospinning.
- To create a platform for optical oxygen transduction with enzyme immobilization.
- To demonstrate the material's utility for determining uric acid concentrations.
Main Methods:
- Co-electrospinning of coaxial nanofibers with an oxygen-sensitive dye and aldehyde groups.
- Immobilization of uricase enzyme via Schiff base formation.
- Optimization of parameters including temperature, pH, reaction time, buffer, and enzyme concentration.
- Application in optical oxygen transduction for uric acid determination.
Main Results:
- The developed material integrates oxygen-sensing capabilities with high enzyme immobilization capacity.
- Optimized conditions demonstrated the material's versatility for biosensing.
- Successful determination of uric acid in serum samples was achieved.
- The material maintained the optical transducer's oxygen-sensing properties.
Conclusions:
- The novel multifunctional material is a promising platform for biosensing applications.
- Co-axial nanofiber structure facilitates enzyme immobilization and optical oxygen transduction.
- This approach offers a versatile strategy for developing advanced biosensors.
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