Related Experiment Video
Updated: Feb 13, 2026
09:41
Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke
Published on: October 1, 2020
5.8K
Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
Emma V Lampard1, Adam C Sedgwick1, Thitima Sombuttan2
1Department of Chemistry University of Bath BA2 7AY Bath UK.
Chemistryopen
|March 14, 2018
Summary
Benzoxaborole hydrogels (BOBgels) offer improved detection of monosaccharides like glucose. This advancement builds upon earlier boronic acid-Alizarin red S dye displacement assays for fructose determination.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Dye displacement assays are established methods for analyte quantification.
- Boronic acid pinacol ester hydrogels (borogels) were previously utilized for fructose detection via a boronic acid-Alizarin red S assay.
Purpose of the Study:
- To develop novel benzoxaborole hydrogels (BOBgels).
- To evaluate the binding affinity of BOBgels towards monosaccharides, focusing on glucose detection.
Main Methods:
- Synthesis of benzoxaborole hydrogels (BOBgels).
- Utilizing BOBgels in a dye displacement assay format.
- Assessing the apparent binding affinity towards monosaccharides, particularly glucose.
Main Results:
- BOBgels were successfully developed.
- BOBgels exhibited enhanced apparent binding affinity for monosaccharides.
- The assay demonstrated improved sensitivity towards glucose compared to previous methods.
Conclusions:
- Benzoxaborole hydrogels represent a promising advancement in dye displacement assays.
- BOBgels offer superior detection capabilities for monosaccharides like glucose.
- This work expands the utility of boronic acid-based hydrogels in analytical chemistry.
Related Concept Videos
01:19Displacement Current
3.8K
Ampère's law, in its usual form, does not work in places where the current changes with time and is not steady. Thus, Maxwell suggested including an additional contribution, called the displacement current, Id, to the real conduction current I.
3.8K
01:31Position and Displacement
27.1K
The position of an object defines its location relative to a convenient frame of reference at any particular time. A frame of reference is an arbitrary set of axes from which the position and motion of an object are described. Earth is often used as a frame of reference, and we often describe the position of an object as it relates to stationary objects on Earth. For example, a rocket launch could be described in terms of the position of the rocket with respect to Earth as a whole. On the other...
27.1K
01:00Position and Displacement Vectors
13.9K
To describe the motion of an object, one should first be able to describe its position (where it is at any particular time). More precisely, the position needs to be specified relative to a convenient frame of reference. A frame of reference is an arbitrary set of axes from which the position and motion of an object are described. Earth is often used as a frame of reference to describe the position of an object in relation to stationary objects on Earth.
Further, several important kinds of...
Further, several important kinds of...
13.9K
01:27Significance of Displacement Current
6.0K
A displacement current is analogous to a real current in Ampère's law, participating in Ampère's law the same way as the usual conduction current. However, it is produced by a changing electric field. Displacement current is defined in terms of a time-varying electric field, and also has an associated displacement current density. By adding a term accounting for displacement current, Maxwell modified the existing Ampère's law, which is now called generalized Ampère's law.
6.0K
01:08Angular Velocity and Displacement
22.9K
Uniform circular motion is motion in a circle at a constant speed. Although this is the simplest case of rotational motion, it is very useful for many situations and is used to introduce rotational variables. When a particle is moving in a circle, the coordinate system is fixed and serves as a frame of reference to define the particle’s position. Its position vector from the origin of the circle to the particle sweeps out the angle θ, which increases in the counterclockwise direction...
22.9K
01:30EDTA: Direct, Back-, and Displacement Titration
5.6K
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
5.6K