Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.7K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.1K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.1K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.4K
3.4K
pre-mRNA Processing02:01

pre-mRNA Processing

57.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.5K
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zanubrutinib, Obinutuzumab, and Venetoclax in CLL: Long-Term Follow Up, MRD Kinetics, Retreatment, T-Cell Profiling, PKs.

Blood advances·2026
Same author

Loss of miR-146a-5p contributes to ibrutinib resistance in mantle cell lymphoma.

Blood advances·2026
Same author

A U1-U3 snRNA-snoRNA interaction couples SF3B1 mutation to chromatin-state rewiring and genome instability.

bioRxiv : the preprint server for biology·2026
Same author

Outcomes of Adolescents and Young Adults with AML Treated on Pediatric vs Adult Protocols.

Blood advances·2026
Same author

Non-enzymatic RNA Glycation is a Metabolic Sensor of Cellular Stress.

bioRxiv : the preprint server for biology·2026
Same author

Molecular and therapeutic frontiers in anemia therapy.

The Journal of clinical investigation·2026
Same journal

Circadian Homeostasis of Liver Metabolism Suppresses Hepatocarcinogenesis.

Cancer cell·2026
Same journal

Vascular RhoJ Is an Effective and Selective Target for Tumor Angiogenesis and Vascular Disruption.

Cancer cell·2026
Same journal

Intratumoral B cells under stress.

Cancer cell·2026
Same journal

Chronic stress unleashes an intratumor phage-fibroblast-B cell circuit to promote tumor growth.

Cancer cell·2026
Same journal

Molecular phenotypes and spatial archetypes: A new framework for cancer-associated fibroblasts.

Cancer cell·2026
Same journal

OpenIO: An open framework for AI-native immunotherapy.

Cancer cell·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.6K

Targeting mRNA Decapping in AML.

Akihide Yoshimi1, Omar Abdel-Wahab2

  • 1Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center and Weill Cornell Medical College, New York, NY, USA.

Cancer Cell
|March 14, 2018
PubMed
Summary
This summary is machine-generated.

Researchers discovered that acute myeloid leukemia (AML) depends on an enzyme called DCPS, which is crucial for RNA metabolism. This enzyme can be targeted with a drug, offering a potential new treatment strategy for AML patients.

More Related Videos

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
06:46

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Published on: November 9, 2019

24.1K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.6K

Related Experiment Videos

Last Updated: Feb 13, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.6K
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
06:46

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Published on: November 9, 2019

24.1K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.6K

Area of Science:

  • Molecular Biology
  • Cancer Research
  • RNA Metabolism

Background:

  • Acute myeloid leukemia (AML) is a complex hematological malignancy.
  • RNA metabolism plays a critical role in cellular function and disease pathogenesis.
  • The enzyme decapping enzyme (DCPS) is involved in messenger RNA (mRNA) degradation.

Purpose of the Study:

  • To investigate the role of DCPS in acute myeloid leukemia.
  • To determine if DCPS is a potential therapeutic target in AML.

Main Methods:

  • Utilized molecular biology techniques to study DCPS function in AML cell lines.
  • Employed a clinical inhibitor of DCPS to assess its efficacy in preclinical AML models.

Main Results:

  • Identified a significant dependency of AML cells on DCPS activity.
  • Demonstrated that inhibiting DCPS effectively reduces AML cell viability and proliferation.
  • Confirmed that DCPS is a druggable target in AML.

Conclusions:

  • DCPS is essential for the survival and proliferation of acute myeloid leukemia cells.
  • Targeting DCPS with a clinical inhibitor presents a promising therapeutic strategy for AML.
  • This discovery highlights the translational potential of targeting RNA metabolism in cancer therapy.