Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

5.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.6K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

4.1K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.1K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

5.4K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
5.4K
pH Regulation in Cells01:28

pH Regulation in Cells

7.8K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
7.8K
Elasticity01:12

Elasticity

5.0K
Elasticity is the ability of an object to withstand the effects of distortion and to return to its original size and shape once the forces causing deformation are removed. When an elastic material deforms under the action of an external force, it experiences internal resistance to the deformation. However, if no external force is applied, it returns to its original state.
The elasticity of an object can be described by a stress-strain curve, which represents the relationship between stress...
5.0K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MMP9 drives cancer invasion by mediating integrin membrane trafficking and stabilization.

The FEBS journal·2026
Same author

Mechanistic insights into Bluetongue virus immunodynamics: a Bayesian within-host modelling approach.

Journal of the Royal Society, Interface·2026
Same author

A functional placenta-on-chip model for maternal-fetal transport.

Biofabrication·2026
Same author

Differential Expression of LncRNA NEAT1 in 3D Tumoroids Compared to 2D Cultures Highlights Its Role in Glioblastoma Progression.

Macromolecular bioscience·2026
Same author

Synthetic melanin as a bioinspired antioxidant: Modulating oxidative stress from 2D skin models to human skin validation.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Matrix stiffness and stress relaxation regulate osteogenesis through histone demethylases KDM4B and KDM6B.

Molecular biology of the cell·2026

Related Experiment Video

Updated: Feb 13, 2026

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
09:10

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

3.7K

Matrix elasticity regulates mesenchymal stem cell chemotaxis.

Neha Saxena1, Pankaj Mogha1, Silalipi Dash1

  • 1Department of Chemical Engineering, IIT, Bombay, Maharashtra 400076, India.

Journal of Cell Science
|March 15, 2018
PubMed
Summary

Human mesenchymal stem cell (hMSC) homing efficiency depends on both physical and chemical cues. Softer substrates enhance hMSC chemotaxis by promoting weaker adhesions and faster protrusions, crucial for cell migration.

Keywords:
ChemotaxisMesenchymal stem cellsMicrofluidicsStiffness

More Related Videos

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
09:00

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

28.5K
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
16:55

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

Published on: October 26, 2011

20.9K

Related Experiment Videos

Last Updated: Feb 13, 2026

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
09:10

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

3.7K
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
09:00

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

28.5K
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
16:55

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

Published on: October 26, 2011

20.9K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Stem Cell Research

Background:

  • Efficient homing of human mesenchymal stem cells (hMSCs) is critical for therapeutic applications.
  • The interplay between physical (e.g., substrate stiffness) and chemical (e.g., growth factors) microenvironmental cues governing hMSC migration is not fully understood.

Purpose of the Study:

  • To investigate the combined effects of substrate stiffness and epidermal growth factor (EGF) concentration on hMSC chemotaxis.
  • To elucidate the mechanisms underlying hMSC migration in response to physical and chemical stimuli.

Main Methods:

  • Utilized a polydimethylsiloxane (PDMS) microfluidic device to test hMSC chemotaxis on substrates with varying stiffness (3, 30, and 600 kPa).
  • Measured chemotactic speed and analyzed cellular responses, including adhesion, protrusion rates, and the role of the actin cytoskeleton.
  • Investigated the impact of contractility inhibitors and actin depolymerization on hMSC migration.

Main Results:

  • hMSC chemotactic speed is an additive function of stiffness-dependent and chemokine concentration-dependent components.
  • Chemotaxis speed increased on softer substrates (inversely proportional to stiffness), attributed to reduced cell adhesion and increased protrusion rates.
  • Actin-mediated protrusions play a crucial role in driving hMSC chemotaxis, as evidenced by migration suppression upon actin depolymerization.

Conclusions:

  • Both physical substrate properties and chemical signals collectively influence hMSC chemotactic migration.
  • Softer microenvironmental substrates enhance hMSC homing efficiency, suggesting implications for optimizing cell delivery in regenerative medicine.
  • Understanding these combined cues is vital for engineering environments that promote effective stem cell homing.