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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
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Transcriptome Sequencing: RNA-Seq.

Hong Zhang1, Lin He1, Lei Cai2

  • 1Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center for Genetics and Development, Shanghai Jiaotong University, Shanghai, 200240, China.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2018
PubMed
Summary
This summary is machine-generated.

RNA sequencing (RNA-seq) identifies gene expression and abnormalities like alternative splicing. Different next-generation sequencing (NGS) platforms require specific kits for accurate RNA-seq library preparation.

Keywords:
Data analysisLibrary constructionMessenger RNANext-generation sequencingRNA sequencing

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • RNA sequencing (RNA-seq) is a powerful tool for transcriptomic analysis.
  • It enables the detection of gene expression levels for both common and rare transcripts.

Purpose of the Study:

  • To highlight the versatility of RNA sequencing beyond basic expression analysis.
  • To discuss the technical considerations for RNA-seq library preparation across different platforms.

Main Methods:

  • RNA sequencing (RNA-seq) utilizes next-generation sequencing (NGS) technologies.
  • Library preparation protocols are tailored to specific NGS instrument requirements.

Main Results:

  • RNA-seq facilitates the identification of alternative splicing events.
  • Novel transcripts and fusion genes can be discovered using RNA-seq.
  • Platform-specific kits are essential for successful RNA-seq library construction.

Conclusions:

  • RNA-seq is a comprehensive method for exploring various aspects of the transcriptome.
  • Understanding platform-specific library preparation is crucial for robust RNA-seq experiments.