Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.4K
Overview
80.4K
Interference and Diffraction02:18

Interference and Diffraction

52.6K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.6K
RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PE-STAR: prime editing with SOS-triggered and RecJ-augmented repair enables high-efficiency editing in Escherichia coli.

Nucleic acids research·2026
Same author

Engineering <i>Escherichia coli</i> for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates.

Synthetic and systems biotechnology·2026
Same author

Integrated dynamic control and enzyme co-localization strategies enable high-efficiency stilbenoid biosynthesis.

Bioresource technology·2026
Same author

Engineering a High-Fidelity MAD7 Variant with Enhanced Specificity for Precision Genome Editing via CcdB-Based Bacterial Screening.

Biomolecules·2025
Same author

SELECT: high-precision genome editing strategy via integration of CRISPR-Cas and DNA damage response for cross-species applications.

Nucleic acids research·2025
Same author

Emerging gene editing in industrial microbiology beyond CRISPR-Cas9.

Trends in biotechnology·2024
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

7.0K

Combinatorial pathway engineering using type I-E CRISPR interference.

Katia Tarasava1, Rongming Liu2, Andrew Garst3

  • 1Chemical and Biological Engineering Department, University of Colorado Boulder, Boulder, Colorado.

Biotechnology and Bioengineering
|March 15, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a novel CRISPR interference method to rapidly generate diverse gene expression libraries for metabolic engineering. The approach significantly boosts production of valuable compounds like 3-hydroxypropionate (3HP).

Keywords:
3-hydroxypropionic acidCRISPR interferencecombinatorial gene expressionmalonyl-coAmetabolic flux engineering

More Related Videos

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.7K
Production of Human CRISPR-Engineered CAR-T Cells
06:33

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

14.7K

Related Experiment Videos

Last Updated: Feb 13, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

7.0K
Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.7K
Production of Human CRISPR-Engineered CAR-T Cells
06:33

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

14.7K

Area of Science:

  • Synthetic Biology
  • Metabolic Engineering
  • CRISPR Technology

Background:

  • Optimizing metabolic flux in complex networks is challenging, often requiring extensive trial-and-error gene expression tuning.
  • Existing methods for generating combinatorial gene expression libraries are limited in scale and efficiency.

Purpose of the Study:

  • To develop an efficient method for generating large-scale combinatorial gene expression libraries.
  • To engineer enhanced metabolic flux for increased production of malonyl-CoA and 3-hydroxypropionate (3HP).

Main Methods:

  • Utilized a modified Escherichia coli Type I-E CRISPR-Cas system for gene regulation.
  • Employed an iterative cloning strategy to construct large arrays of guide RNAs (gRNAs).
  • Generated and screened a library of approximately 12,000 combinatorial gene expression variants targeting six genes.

Main Results:

  • Created a combinatorial gene expression library three orders of magnitude larger than previously reported.
  • Identified variants with significantly increased malonyl-CoA flux.
  • Achieved up to a 98% increase in 3-hydroxypropionate (3HP) production.

Conclusions:

  • The developed CRISPR interference method offers a fast and scalable approach for metabolic engineering.
  • This strategy facilitates the development of industrially relevant microbial strains and aids in fundamental biological research.