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Updated: Feb 13, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Detection and genetic characterization of enterovirus strains circulating among children with acute gastroenteritis
Ngan Thi Kim Pham1, Aksara Thongprachum1, Quang Duy Trinh1
1Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Abstract:
Of 972 stool samples collected from infants and children with acute gastroenteritis in six different regions (Hokkaido, Tokyo, Shizuoka, Kyoto, Osaka, and Saga) of Japan during 2-year period from July 2014 to June 2016, 63 samples (6.5%) determined to be positive for enterovirus by multiplex RT-PCR were subjected to genotype determination based on the partial VP1 region using the CODEHOP method. Sixty-two strains were succeeded in genotyping and seventeen EV types were identified. The majority of the studied strains belonged to EV-A (30 of 62; 48.4%) and EV-B (31 of 62, 50%) species, and the remaining strain was of the EV-D species. The most frequently detected type was Coxsackievirus A5 (CV-A5) in 2014-2015 while was CV-B5 in 2015-2016. This study provides an insight into the genetic diversity of EV with the predominance of EV-A and EV-B species in Japanese infants and children with acute gastroenteritis during 2014-2016.
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