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A Practical Guide to Phylogenetics for Nonexperts
Published on: February 5, 2014
Genomic characterization and phylogenetic analysis of the novel Pseudomonas phage PPSC2
Xiang Wu1,2, Yongfeng Wu3, Ya Tang4
1College of Architecture and Environment, Sichuan University, No. 24, south section of 1st Ring Road, Chengdu, 610065, China.
Abstract:
We isolated a Pseudomonas phage infecting Pseudomonas fluorescens SA1 separated from a soil sample collected in Sichuan Province, China. This phage, which we named PPSC2, has a genome that is composed of a 97,330-bp-long linear double-stranded DNA with 47.51% G+C content and 168 putative protein-coding genes. We identified 20 tRNA genes in the genome of PPSC2, and the tRNA GC content ranged from 44.2% to 58.4%. Phylogenetic and BLASTn analysis revealed that the Pseudomonas phage PPSC2 should be considered a new member of the family Myoviridae.
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