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Updated: Feb 13, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Fabrication of SrTiO₃ Layer on Pt Electrode for Label-Free Capacitive Biosensors
Francesca Malvano1, Luigi Maritato2, Giovanni Carapella3
1Department of Industrial Engineering, University of Salerno, 84084 Fisciano, Italy. fmalvano@unisa.it.
Abstract:
Due to their interesting ferroelectric, conductive and dielectric properties, in recent years, perovskite-structured materials have begun to attract increasing interest in the biosensing field. In this study, a strontium titanate perovskite layer (SrTiO₃) has been synthesized on a platinum electrode and exploited for the development of an impedimetric label-free immunosensor for Escherichia coli O157:H7 detection. The electrochemical characterization of the perovskite-modified electrode during the construction of the immunosensor, as well as after the interaction with different E. coli O157:H7 concentrations, showed a reproducible decrease of the total capacitance of the system that was used for the analytical characterization of the immunosensor. Under optimized conditions, the capacitive immunosensor showed a linear relationship from to 1 to 7 log cfu/mL with a low detection limit of 1 log cfu/mL. Moreover, the atomic force microscopy (AFM) technique underlined the increase in roughness of the SrTiO₃-modified electrode surface after antibody immobilization, as well as the effective presence of cells with the typical size of E. coli.
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