Fluorescent microplate assay method for high-throughput detection of lipase transesterification activity
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Updated: Feb 13, 2026

A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
Published on: June 14, 2021
Jianyong Zheng1, Wei Wei1, Xing Lan1
1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hang Zhou 310014, Zhejiang, People's Republic of China.
Abstract:
This study describes a sensitive and fluorescent microplate assay method to detect lipase transesterification activity. Lipase-catalyzed transesterification between butyryl 4-methyl umbelliferone (Bu-4-Mu) and methanol in tert-butanol was selected as the model reaction. The release of 4-methylumbelliferone (4-Mu) in the reaction was determined by detecting the fluorescence intensity at λex 330 nm and λem 390 nm. Several lipases were used to investigate the accuracy and efficiency of the proposed method. Apparent Michaelis constant (Km) was calculated for transesterification between Bu-4-Mu and methanol by the lipases. The main advantages of the assay method include high sensitivity, inexpensive reagents, and simple detection process.
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