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Evaluation of the Storage Stability of Extracellular Vesicles
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PIBF+ extracellular vesicles from mouse embryos affect IL-10 production by CD8+ cells
Eva Pallinger1, Zoltan Bognar2,3,4, Agnes Bogdan2,5,4
1Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Scientific Reports
|March 18, 2018
Summary
The embryo communicates with the maternal immune system using extracellular vesicles (EVs). These embryo-derived EVs increase the number of IL-10+ CD8+ T cells, suggesting a role in immune modulation.
Area of Science:
- Reproductive immunology
- Cellular and molecular biology
- Immunology
Background:
- The maternal immune system undergoes adaptations during pregnancy.
- Intercellular communication via extracellular vesicles (EVs) is crucial in biological processes.
- Embryo-maternal communication is vital for successful pregnancy.
Purpose of the Study:
- To investigate if embryo-derived extracellular vesicles (EVs) can modulate maternal peripheral lymphocyte function.
- To determine the role of progesterone-induced blocking factor (PIBF) in embryo-derived EVs mediating immune responses.
Main Methods:
- Identification and characterization of embryo-derived EVs using flow cytometry and transmission electron microscopy.
- Detection of PIBF expression on EVs via immuno-electron microscopy.
- Quantification of IL-10+ murine lymphocytes using flow cytometry.
Main Results:
- Embryo-derived EVs were detected in culture media and at the implantation site.
- Embryo-derived EVs bind to murine CD4+ and CD8+ T lymphocytes.
- The presence of embryo-derived EVs increased IL-10+ CD8+ T cells, an effect blocked by anti-PIBF antibodies.
Conclusions:
- Embryo-derived EVs are involved in intercellular communication with the maternal immune system.
- PIBF expressed on embryo-derived EVs plays a role in modulating CD8+ T cell responses.
- EVs represent a significant mechanism for embryo-maternal immune signaling.
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