Related Experiment Video
Updated: Feb 13, 2026
09:49
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
Published on: November 8, 2014
13.0K
Novel enzymatic method for assaying Lp-PLA2 in serum
Saki Yamaura1, Shin-Ichi Sakasegawa1, Emisa Koguma1
1Asahi Kasei Pharma Corporation, Shizuoka 410-2321, Japan.
Summary
A new enzymatic assay measures lipoprotein-associated phospholipase A2 (Lp-PLA2) activity. This method offers a simpler and more efficient way to assess cardiovascular risk compared to existing assays.
Area of Science:
- Biochemistry
- Enzymology
- Cardiovascular Diagnostics
Background:
- Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a marker for cardiovascular event risk.
- Current Lp-PLA2 measurement methods include protein quantification (ELISA) and activity assays using various substrates.
- An enzymatic Lp-PLA2 activity assay using 1-O-Hexadecyl-2-acetyl-rac-glycero-3-phosphocholine (rac C16 PAF) was developed.
Purpose of the Study:
- To develop a novel enzymatic assay for measuring Lp-PLA2 activity.
- To evaluate the performance of the new assay against existing methods.
Main Methods:
- The assay exploits the substrate specificity of lysophospholipase D (LysoPLD).
- Lp-PLA2 hydrolyzes C16 PAF to LysoPAF, which is then acted upon by LysoPLD.
- Released choline is detected using choline oxidase.
Main Results:
- The new enzymatic assay showed high correlation with chemical Lp-PLA2 assays (r=0.990 for LpPLA2 FS, r=0.893 for PLAC® test).
- Correlation with ELISA was also significant (r=0.785).
Conclusions:
- The developed enzymatic Lp-PLA2 activity assay is a simple and effective method.
- Advantages include a two-reagent system, a simple calibration method, and no requirement for serum esterase inhibitors.
Keywords:
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholineEnzymatic assay methodLp-PLA(2)Lysophospholipase DPAFPAF-AH (platelet-activating factor-acetylhydrolase)More Related Videos
Related Concept Videos
01:22Amplifying Signals via Enzymatic Cascade
18.6K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
18.6K
01:24Serum Studies: Renal Function Tests
457
Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
457
01:30Serum Laboratory Studies, Stool Test, Breath Test
893
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
893
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
645
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
645
01:25Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance
233
In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance...
233
01:29Methods of Sterilization I: Physical Methods
23.8K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
23.8K