Related Experiment Video
Updated: Feb 13, 2026

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
Published on: April 11, 2013
Endophytic biocatalysts with enoate reductase activity isolated from Mentha pulegium
Facundo Marconi1, María Laura Umpiérrez2, David Gonzalez1
1Laboratorio de Biocatálisis y Biotransformaciones, Departamento de Biociencias y Departamento de Química Orgánica, Universidad de la República, Gral. Flores 2124, 11800, Montevideo, Uruguay.
Abstract:
The biotransformation of (4R)-(-)-carvone by Mentha pulegium (pennyroyal) leaves and its endophytic bacteria was performed in order to search for novel biocatalysts with enoate reductase activity. The obtained results clearly indicated that endophytes play an important role in the biotransformation of (4R)-(-)-carvone with pennyroyal plant tissues. The best activity was associated to the endophytic bacteria Pseudomonas proteolytica FM18Mci1 and Bacillus sp. FM18civ1. Enoate reductase activity for the reduction of (4R)-(-)-carvone and (4S)-(+)-carvone as model substrates was evaluated for each strain. Finally, both isolated strains were evaluated for the kinetic resolution of racemic carvone. The two bacteria gave (1R, 4R) or (1R, 4S)-dihydrocarvone as major products. P. proteolytica FM18Mci1 had preference for the 4S-(-)-carvone, reaching a conversion 95% in 24 h. In contrast, Bacillus sp. FM18civ1 had preference for (4R)-(-)-carvone. The results obtained in the kinetic resolution of carvone indicated that the Bacillus strain could be useful for resolving a racemic mixture of carvone.
More Related Videos
Related Concept Videos
Co-activators and Co-repressors
tRNA Activation
Activation Energy
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
Secondary Active Transport
Primary Active Transport

