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Related Experiment Video

Updated: Feb 13, 2026

$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

A Rare Lysozyme Crystal Form Solved Using Highly Redundant Multiple Electron Diffraction Datasets from Micron-Sized

Hongyi Xu¹, Hugo Lebrette², Taimin Yang¹

¹Inorganic and Structural Chemistry, Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden.

Structure (London, England : 1993)

|March 20, 2018

Summary

This summary is machine-generated.

Novel electron diffraction (MicroED) successfully determined the atomic structure of a rare lysozyme polymorph from tiny crystals. Merging data from multiple crystals enhanced structural quality, proving MicroED

Keywords:

MicroED continuous rotation electron diffraction cryo-EM electron crystallography electron diffraction lysozyme macromolecular structure protein crystallography protein structure transmission electron microscopy

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Area of Science:

Crystallography
Electron Microscopy
Biophysics

Background:

Single-crystal X-ray diffraction is limited to larger crystals.
Micron- and nano-sized crystals are challenging for traditional methods.
Electron diffraction techniques offer new possibilities for small crystals.

Purpose of the Study:

To solve and refine the atomic structure of a rare lysozyme polymorph.
To demonstrate the utility of continuous rotation MicroED for small crystal structure determination.
To assess the impact of merging data from multiple crystals on structural quality.

Main Methods:

Microcrystal Electron Diffraction (MicroED) data collection using a 200 kV transmission electron microscope (TEM) with a sensitive, fast-readout detector.
Continuous rotation data acquisition.
Structure refinement using standard X-ray crystallographic software.
Merging datasets from 33 individual crystals.

Main Results:

The atomic structure of a rare lysozyme polymorph was successfully solved and refined.
Data merging from 33 crystals significantly improved data completeness, I/σ, and redundancy.
Enhanced data quality led to a high-quality final atomic model.
MicroED data collection was rapid (approx. 3 minutes per crystal).

Conclusions:

Continuous rotation MicroED is a powerful technique for determining atomic-resolution structures from small crystals.
Merging data from multiple small crystals is effective for improving structural model quality.
This method is particularly valuable for electron beam-sensitive crystals or those with preferred orientation.