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Updated: Feb 13, 2026

Investigating the Phagocytosis of Leishmania using Confocal Microscopy
Published on: July 29, 2021
Leishmania donovani asparaginase variants exhibit cytosolic localization
Kartikeya Tiwari1, Vikash Kumar Dubey1
1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam 781039, India.
Abstract:
Asparaginase, a pivotal enzyme of the aspartate metabolic pathway is present as two variants in Leishmania donovani, causative agent of visceral leishmaniasis (VL). Our microscopy based analysis revealed that the green fluorescent protein tagged asparaginase variants were localized in the cytosol of the parasite. The finding was also supported by the in silico analysis of the L. donovani asparaginase variants. This finding is in stark contrast with other parasitic species such as M. tuberculosis where asparaginase is secreted out of the parasite. Further studies are required to decipher the exact function of asparaginase variants in the infectivity and growth of the parasite.
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