Interspecific Variation in Coral Settlement and Fertilization Success in Response to Hydrogen Peroxide Exposure

Hydrogen peroxide (H₂O₂) is involved in the regulation of numerous reproductive and morphogenic processes across an array of taxa. Extracellular H₂O₂ can be widespread in oceanic waters, and elevated sea surface temperatures can cause increased levels of intracellular H₂O₂ within cnidarian tissue, but it remains unclear how this compound affects early life-history processes in corals, such as fertilization, metamorphosis, and settlement. To evaluate the effects of H₂O₂ on multiple stages of recruitment, experiments were conducted using Caribbean corals with various reproductive modes, including the brooders Porites astreoides and Favia fragum and the broadcast-spawning species Acropora palmata and Orbicella franksi. H₂O₂ accelerated settlement in all brooding species tested. Concentrations of 1000 µmol l^-1 H₂O₂ caused close to 100% settlement in all larval age classes, regardless of exposure duration. As larvae aged, the required threshold of H₂O₂ capable of inducing settlement decreased. In contrast, H₂O₂ concentrations of 100 µmol l^-1 or greater caused a significant reduction in metamorphosis and settlement in the larvae of spawners. Furthermore, fertilization of their gametes was inhibited in the presence of H₂O₂ concentrations as low as 100 µmol l^-1. In Porites astreoides larvae, internal levels of H₂O₂ reached a maximal value of 75 µmol l^-1 following 48 h of incubation at 31 °C. This concentration was found to significantly alter settlement rates in both brooding coral species and likely induced a cellular cascade in the settlement signaling pathway. The results of this study suggest that temperature stress influences H₂O₂ production, which in turn impacts coral settlement. While it is unlikely that the current levels of externally derived concentrations of oceanic H₂O₂ are affecting coral larvae, internal concentrations (produced under heat stress) have the capacity to impact recruitment under a changing climate.