Reactivation of streptolysin S by oligonucleotide

Oligonucleotide-streptolysin S complex inactivated by alkali treatment remains nonhemolytic, even after acidification and mixing with intact carrier oligonucleotide rich in guanyl residue. Upon dehydration, however, the inactive streptolysin S-oligonucleotide mixture turned to be hemolytic, and this reactivation of the hemolysin was promoted by treatment with guanidine hydrochloride. After alkaline hydrolysis, streptolysin S was freed from nucleotide moiety, by gel filtration through a Sephadex G-50 column. From this nonhemolytic apotoxin as well, active streptolysin S complex was reconstructed upon dehydration with the carrier.