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Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
Evaluation of calcium and magnesium contents in tooth enamel without any pathological changes: in vitro preliminary
Elzbieta Klimuszko1, Karolina Orywal2, Teresa Sierpinska3
1Department of Prosthetic Dentistry, Medical University of Bialystok, M. Sklodowska-Curie Str. 24a, 15-276, Bialystok, Poland.
Abstract:
Enamel is the structure that covers the entire clinical crown of a tooth. It enables to chew and crush food, and gives a final shape to the crowns of teeth. To evaluate calcium and magnesium contents in tooth enamel and analyse relationships between the study minerals extracted human permanent teeth were cut at every 150 microns and subjected into acid biopsy. The amounts of calcium and magnesium were assessed in the laboratory using atomic absorption spectroscopy with an air/acetylene flame. The lowest calcium and magnesium contents were found on the enamel surface of the teeth. Statistically significant correlation between the calcium and magnesium concentrations was found at a depth between 150 and 900 µm. Calcium and magnesium contents increased with increasing enamel depth. Calcium and magnesium deposits appeared to be stable through all the enamel layers studied. It would be suggested that mineralization/demineralization affect only external layer of the enamel, whereas deeper layers are not affected by these processes.
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