Multiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures

Current multiplexed analysis methods suffer from either slow reaction kinetics (planar arrays) or complicated encoding/decoding procedures (suspension arrays). We report herein a multiplexed DNA detection strategy that addresses these issues, based on positional encoding/decoding with self-assembled DNA nanostructures. The strategy enables the acquisition of high-resolution, consistent, and quantitative assay results in a single round of a transmission electron microscopy imaging operation. Applications in polymerase chain reaction-free settings and assays of other structurally distinct targets can be anticipated through the implementation of the strategy with miniaturized femtoliter/attoliter dispensing technology and readily accessible DNA conjugate structures.