Deinococcus rufus sp. nov., isolated from soil near an iron factory

A Gram-stain-negative, non-motile, rod-shaped, red-pigmented strain, designated W37^T, was isolated from soil near an iron factory in Busan (Republic of Korea). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W37^T was most closely related to Deinococcus yunweiensis YIM 007^T (98.3 %) and Deinococcus radioresistens 8A^T (96.3 %). The DNA-DNA relatedness between strain W37^T and D. yunweiensis YIM 007^T was 50.5 %. The predominant respiratory quinone was MK-8. The major polar lipids were an unidentified phosphoglycolipid, an unidentified aminophospholipid, four unidentified glycolipids, two unidentified phospholipids and an unidentified lipid. The major fatty acids (>5 %) of strain W37^T were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C17 : 1ω8c and iso-C17 : 1ω9c. The DNA G+C content was 69.0 mol%. Moreover, the chemo-physical characteristics of strain W37^T clearly differed from those of related species, including ranges of growth temperature and pH, positive activity for 4-hydroxybenzoate and negative activity for cystine arylamidase. Phenotypic, chemotaxonomic and genotypic analyses indicated that strain W37^T represents a novel species of the genus Deinococcus, for which the name Deinococcus rufus sp. nov., is proposed. The type strain is W37^T (=KCTC 33913^T=CCTCC AB 2017081^T).