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Updated: Feb 13, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
Immunization with HER2 extracellular subdomain proteins induces cellular response and tumor growth inhibition in mice
Moslem Ahmadi1,2, Fateme Sadri-Ardalani1,2, Mohammad M Amiri1
1Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Aim:
We investigated cellular and protective immune responses in mice vaccinated with recombinant HER2 extracellular subdomains.
Materials & Methods:
Balb/C mice were immunized with recombinant full HER2 extracellular domain and subdomain proteins. Humoral and cellular immune response and antitumor effect was evaluated using a syngeneic mice tumor model.
Results:
All recombinant proteins induced secretion of IL-4 and particularly IFN-γ and IL-17 cytokines. Challenging of immunized mice with stable 4T1-HER2 transfected cells resulted in partial but significant tumor growth inhibition in all groups of mice particularly those immunized with fHER2-ECD together with CPG.
Conclusion:
Our results suggest that the recombinant HER2-ECD subdomains induce mainly Th1 and Th17 responses, which seem to contribute to tumor growth inhibition in syngeneic mice.
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