Related Experiment Video
Updated: Feb 12, 2026

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
Published on: December 19, 2016
Isolation and Culture of Mouse Placental Endothelial Cells
Lijun Chi1, Paul Delgado-Olguin2,3,4
1Translational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
Abstract:
Isolation and culture of endothelial cells (ECs) is a useful tool to study the cellular processes involved in vascular development and vascular maturation. In this chapter, we describe a method to isolate and culture endothelial cells from placentae. This method takes advantage of two transgenes: ROSA26 mT/mG , which drives the expression of GFP upon Cre-mediated recombination, and Tie2-Cre, which expresses Cre driven by the Tie2 promoter in endothelial progenitors and their descendants. GFP-expressing endothelial cells are isolated through fluorescence-activated cell sorting (FACS). The sorted cells express the endothelial marker CD31. This method can be used to study the morphological and physiological properties of placental endothelial cells in mice carrying mutations affecting vascular development.
Related Concept Videos
Gonadal and Placental Hormones
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair...
Techniques for Isolation of Pure Cultures
Cell Culture
Stem Cell Culture
Overview Of Cell Separation And Isolation
Plant Tissue Culture

