Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple topogenic sequences in bovine opsin.

Y Audigier, M Friedlander, G Blobel

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Microbiome depletion rejuvenates the aging brain.

    bioRxiv : the preprint server for biology·2026
    Same author

    The impact of olaparib dose reduction and treatment interruption on treatment outcome in the SOLO2/ENGOT-ov21 platinum-sensitive recurrent ovarian cancer.

    Annals of oncology : official journal of the European Society for Medical Oncology·2022
    Same author

    Veliparib monotherapy following carboplatin/paclitaxel plus veliparib combination therapy in patients with germline BRCA-associated advanced breast cancer: results of exploratory analyses from the phase III BROCADE3 trial.

    Annals of oncology : official journal of the European Society for Medical Oncology·2021
    Same author

    Phase 2 study of anastrozole in rare cohorts of patients with estrogen receptor/progesterone receptor positive leiomyosarcomas and carcinosarcomas of the uterine corpus: The PARAGON trial (ANZGOG 0903).

    Gynecologic oncology·2021
    Same author

    Genetic associations of fatigue and other symptoms following breast cancer treatment: A prospective study.

    Brain, behavior, & immunity - health·2021
    Same author

    Phase 2 study of anastrozole in patients with estrogen receptor/progesterone receptor positive recurrent low-grade endometrial stromal sarcomas: The PARAGON trial (ANZGOG 0903).

    Gynecologic oncology·2021
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers investigated bovine opsin

    Area of Science:

    • Molecular biology
    • Membrane protein topology
    • Protein biogenesis

    Background:

    • Bovine opsin is a polytopic integral membrane protein with seven transmembrane segments.
    • Understanding the topogenic sequences that dictate opsin's membrane topology is crucial.

    Purpose of the Study:

    • To identify specific topogenic sequences within bovine opsin that determine its membrane topology.
    • To analyze the function of individual transmembrane segments as signal and stop-transfer sequences.

    Main Methods:

    • Construction and analysis of bovine opsin mutants, each with a single transmembrane segment.
    • In vitro translation of mutant messenger RNAs in a cell-free system with microsomal membranes.
    • Assessing membrane protein integration using extractability at pH 11 and protease sensitivity.

    Related Experiment Videos

    Main Results:

    • Five of the six analyzed transmembrane segments of bovine opsin exhibited both signal sequence and stop-transfer sequence activity.
    • The strength of the stop-transfer sequence, in conjunction with a signal sequence, influenced membrane integration.
    • Strong stop-transfer sequences promoted lipid bilayer integration, while weak ones resulted in varied translocation outcomes.

    Conclusions:

    • Bovine opsin transmembrane segments possess dual functionality as signal and stop-transfer sequences.
    • The interplay between signal and stop-transfer sequence strength dictates the final topology of opsin within the membrane.
    • This study elucidates the mechanisms governing the insertion and topology of polytopic membrane proteins.