Related Experiment Video
Updated: Feb 12, 2026

Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
Comparison Between a Manual Squamous Cell Carcinoma Antigen Assay and an Automated Assay in a Clinical Setting
Saejin Lee1, Juwon Kim1, Young Uh1
1Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea.
Objective:
To compare a manual squamous cell carcinoma antigen (SCCA) assay and an automated SCCA assay in a clinical setting.
Methods:
We included, in this study, a total of 158 specimens that had been tested using the manual SCCA assay. The CanAg SCC EIA assay and the Elecsys SCC were compared for their clinical settings.
Results:
Within-run and between-day coefficients of variation (CVs, %) were lower than 3%. Comparison of the manual and automated SCCA assays yielded good correlation. The correlation coefficient (R2) between the CanAg SCC EIA and Elecsys SCC assay results was 0.989 (P <.001) and the overall concordance rate was 94.3%.
Conclusions:
We report that the Elecsys SCC automated SCCA assay yielded performance comparable to that of the manual SCCA assay: the automated assay reduced the number of manual steps and test turnaround time.
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