Related Experiment Video

Updated: Feb 12, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.4K

Targeting DYRK1B suppresses the proliferation and migration of liposarcoma cells

Hua Chen1,2, Jacson Shen2, Edwin Choy2

  • 1Department of Emergency Surgery, ShenZhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen, Guangdong Province, China, 518020.

Oncotarget
|March 24, 2018
PubMed

Insights

Dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B) drives liposarcoma growth. Inhibiting DYRK1B with AZ191 or doxorubicin shows therapeutic potential for this soft tissue sarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Liposarcoma, a prevalent soft tissue sarcoma, shows limited response to conventional chemotherapy.
  • Serine/threonine-protein kinase dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B) is implicated in various cancer cell growth and survival.
  • The specific role of DYRK1B in liposarcoma pathogenesis and progression is currently undetermined.

Purpose of the Study:

  • To investigate the functional significance of DYRK1B in liposarcoma.
  • To evaluate the therapeutic potential of targeting DYRK1B in liposarcoma models.

Main Methods:

  • Tissue microarray and immunohistochemistry were employed to assess DYRK1B expression levels in liposarcoma tissues.
  • RNA interference-mediated knockdown and the DYRK1B kinase inhibitor AZ191 were used to study DYRK1B inhibition.
  • Cellular assays evaluated the effects on liposarcoma cell proliferation, motility, and apoptosis; combination therapy with doxorubicin was also assessed.

Main Results:

  • Elevated DYRK1B expression levels significantly correlated with poorer prognosis in liposarcoma patients.
  • Inhibition of DYRK1B, via RNA interference or AZ191 treatment, markedly suppressed liposarcoma cell proliferation and motility, while inducing apoptosis.
  • The combination of AZ191 and doxorubicin exhibited enhanced anti-cancer efficacy against liposarcoma cells compared to monotherapy.

Conclusions:

  • DYRK1B plays a critical role in promoting liposarcoma cell growth and survival.
  • Targeting DYRK1B represents a promising novel therapeutic strategy for liposarcoma treatment.
  • Combined inhibition of DYRK1B and conventional chemotherapy may offer improved clinical outcomes for liposarcoma patients.
Keywords:
DYRK1Bapoptosisliposarcomamigrationproliferation

More Related Videos

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines09:57

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Published on: March 5, 2019

10.8K
Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry07:17

Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry

Published on: October 30, 2019

7.3K

Related Concept Videos

Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.7K
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.9K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
5.4K