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Knockdown of long non-coding RNA HOXD-AS1 inhibits the progression of osteosarcoma

Yang Qu1, Shuang Zheng1, Mingyang Kang1

  • 1Department of Orthopaedics, the Second Hospital of Jilin University, #218 Ziqiang Street, Changchun 130041, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|March 25, 2018
PubMed

Insights

Long non-coding RNA HOXD-AS1 is upregulated in osteosarcoma, promoting cancer progression and poor survival. Silencing HOXD-AS1 inhibits tumor growth and metastasis by affecting the STAT3 pathway, suggesting it as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play crucial roles in various cancers.
  • The role of HOXD-AS1 in osteosarcoma (OS) development and progression is currently unknown.

Purpose of the Study:

  • To investigate the expression, significance, and biological function of HOXD-AS1 in osteosarcoma.
  • To explore the potential of HOXD-AS1 as a therapeutic target for OS.

Main Methods:

  • Quantitative real-time PCR to assess HOXD-AS1 expression in OS tissues and cells.
  • In vitro assays (proliferation, colony formation, migration, invasion, cell cycle, apoptosis) to evaluate the function of HOXD-AS1.
  • In vivo xenograft mouse models to assess tumor growth.
  • Western blotting to analyze protein expression (STAT3 and its targets).

Main Results:

  • HOXD-AS1 expression was significantly upregulated in OS tissues and cells.
  • High HOXD-AS1 expression correlated with advanced tumor stage, lymph node metastasis, and reduced overall survival.
  • Knockdown of HOXD-AS1 suppressed OS cell proliferation, migration, invasion, and tumor growth, while promoting apoptosis and G1 cell cycle arrest.
  • HOXD-AS1 knockdown reduced STAT3 and its target protein expression (CyclinD1, Bcl-2, MMP-2).
  • STAT3 overexpression reversed the inhibitory effects of HOXD-AS1 knockdown on proliferation.

Conclusions:

  • HOXD-AS1 acts as an oncogenic lncRNA in osteosarcoma.
  • HOXD-AS1 promotes OS progression through the STAT3 signaling pathway.
  • HOXD-AS1 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
HOXD-AS1OsteosarcomaProliferationSTAT3lncRNA

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