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Identification of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from dogs with pyoderma and
Jae-Eun Hyun1, Tae-Ho Chung2, Cheol-Yong Hwang1
1Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea.
Background:
Pseudomonas aeruginosa is a challenging pathogen cultured from cases of acute and chronic canine otitis and sometimes in cases of deep pyoderma. The spread of antimicrobial resistance, especially carbapenem resistance, is a serious therapeutic challenge worldwide.
Hypothesis/Objectives:
To investigate the identification and characterization of resistant P. aeruginosa clinical canine isolates.
Materials:
Clinical isolates (n = 80) were collected from dogs with pyoderma (n = 18) and otitis (n = 62) in Korea.
Methods:
Antimicrobial susceptibility was determined using agar dilution and using Clinical and Laboratory Standards Institute guidelines for recording susceptibility for human Pseudomonas isolates; genetic relatedness of isolates was investigated by multilocus sequence typing (MLST) and SpeI macrorestriction analysis. The class 1 integrons were amplified and sequenced using primer walking.
Results:
Most isolates were susceptible to colistin (97.5%), polymyxin B (96.3%), ciprofloxacin (81.3%) and meropenem (80.0%); whereas resistance to aztreonam (80%), piperacillin (52.5%), piperacillin/tazobactam (41.3%) and cefepime (37.5%) was high; 12 carbapenem-nonsusceptible isolates (15%) were detected. MLST revealed 45 different sequence types (STs) and macrorestriction analysis detected 55 distinct pulsotypes (PTs), which were divided into 25 clonal groups. Among carbapenem-nonsusceptible isolates, 10 (83.3%) were VIM-2-producing strains. Nine VIM-2-producing isolates were identified as ST1047 and harboured the same 2.8 kb class 1 integron. One remaining isolate was ST1203 with 2.1 kb class 1 integron.
Conclusions And Clinical Importance:
This study demonstrated the diversity of the phenotype and genotype of clinical P. aeruginosa isolates from dogs with pyoderma and otitis. The identification of VIM-2-producing P. aeruginosa in dogs is alarming and warrants further surveillance.
Insights
This study identified carbapenem-resistant Pseudomonas aeruginosa in dogs, with VIM-2 producing strains being a significant concern. Further surveillance is crucial for managing this antimicrobial resistance in canine infections.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance Research
- Canine Infectious Diseases
Background:
- Pseudomonas aeruginosa is a frequent cause of canine otitis and pyoderma.
- Increasing antimicrobial resistance, particularly carbapenem resistance, poses a global therapeutic challenge.
- This study addresses the need to understand resistant P. aeruginosa in veterinary medicine.
Purpose of the Study:
- To identify and characterize antimicrobial-resistant Pseudomonas aeruginosa isolates from clinical canine samples.
- To investigate the genetic diversity and resistance mechanisms of these isolates.
Main Methods:
- Collected 80 clinical P. aeruginosa isolates from dogs with otitis and pyoderma in Korea.
- Determined antimicrobial susceptibility using agar dilution and CLSI guidelines.
- Analyzed genetic relatedness via multilocus sequence typing (MLST) and macrorestriction analysis; sequenced class 1 integrons.
Main Results:
- High resistance observed for aztreonam (80%), piperacillin (52.5%), and piperacillin/tazobactam (41.3%).
- 15% of isolates were non-susceptible to carbapenems; 10 of these (83.3%) produced VIM-2.
- MLST and macrorestriction revealed significant genotypic diversity, with VIM-2 producers mainly belonging to ST1047.
Conclusions:
- Demonstrated considerable phenotypic and genotypic diversity among clinical P. aeruginosa canine isolates.
- The presence of VIM-2 producing P. aeruginosa in dogs is a significant finding requiring attention.
- Highlights the urgent need for enhanced surveillance of resistant P. aeruginosa in veterinary settings.
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