Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Conservation Biology?01:57

What is Conservation Biology?

24.4K
Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.4K
Biological Effects of Radiation02:59

Biological Effects of Radiation

18.1K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
18.1K
Aggregates Classification01:29

Aggregates Classification

1.1K
Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
Petrographic classification groups aggregates based on common mineralogical characteristics. Some of the common mineral groups found in aggregates are...
1.1K
Synthetic Biology02:55

Synthetic Biology

5.6K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.6K
Biological Causes of Schizophrenia01:29

Biological Causes of Schizophrenia

656
Schizophrenia, a severe psychiatric disorder, arises from a complex interplay of biological factors, including genetic predisposition, structural brain abnormalities, neurotransmitter dysregulation, and developmental irregularities. These factors collectively contribute to the onset and progression of the disorder, which typically manifests in late adolescence or early adulthood.
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
656
Bonding and Strength of Aggregate01:12

Bonding and Strength of Aggregate

519
The bond between aggregate particles and the cement matrix is significantly influenced by the shape and surface texture of the aggregates. High-strength concretes benefit from a rougher texture, which leads to stronger bonding due to greater adhesion. Angular aggregates with larger surface areas also enhance this bond. The bonding quality, however, is complex to assess as no universally accepted test exists. Good bonding is indicated when a crushed concrete specimen shows some aggregate...
519

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations of the C-Reactive Protein-Albumin-Lymphocyte Index, Red Cell Distribution Width-Albumin Ratio, and Blood Urea Nitrogen-Albumin Ratio With All-Cause and Cardiovascular Mortality Among Adults With Preclinical Heart Failure.

Clinical cardiology·2026
Same author

A Molecular Engineering Strategy to Fine-Tune Phototoxicity of AIE Probes for Super-Resolution Imaging of Mitochondrial Cristae Dynamics.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Nitric Oxide Conjugation Transforms NIR-II AIEgens Into Potent Hypoxia-Tolerant Type I Photosensitizers.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Purely organic room-temperature phosphorescence sensitizers for OLEDs.

Chemical science·2026
Same author

Molecular conformation engineering in central 8π-electron system toward unique aggregation-induced ultra-narrowband emission with a FWHM of 13 nm.

Light, science & applications·2026
Same author

A Multifunctional Near-Infrared Platinum(II) Agent for High-Performance Chemo-Photothermal Therapy.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Feb 12, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

15.1K

Fluorogenic Ag+ -Tetrazolate Aggregation Enables Efficient Fluorescent Biological Silver Staining.

Sheng Xie1,2, Alex Y H Wong2, Ryan T K Kwok1

  • 1Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science., The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.

Angewandte Chemie (International Ed. in English)
|March 26, 2018
PubMed
Summary

This study introduces a novel fluorescent silver staining method for detecting proteins in gels. This new approach overcomes limitations of traditional silver staining, offering improved sensitivity and reliability for biological research.

Keywords:
aggregation-induced emissionmetal-ion sensorprotein detectionsilver stainingtetrazolate-silver assembly

More Related Videos

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
10:27

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

11.4K
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

5.3K

Related Experiment Videos

Last Updated: Feb 12, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

15.1K
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
10:27

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

11.4K
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

5.3K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Silver staining is a common but limited method for in-gel protein detection.
  • Traditional methods suffer from variability, background noise, and poor quantification.
  • Existing fluorescent stains may also have limitations in sensitivity or dynamic range.

Purpose of the Study:

  • To develop a novel, sensitive, and reliable fluorescent silver staining method for total protein detection in polyacrylamide gels.
  • To overcome the limitations of conventional chromogenic silver staining and existing fluorescent stains.
  • To introduce a new fluorogenic silver probe based on aggregation-induced emission (AIE).

Main Methods:

  • Development of a novel water-soluble fluorogenic silver ion (Ag+) probe.
  • Utilizing an aggregation-induced emission (AIE) mechanism driven by tetrazolate-Ag+ interactions for sensing.
  • Applying the probe for fluorescence-based silver staining of proteins in polyacrylamide gels.

Main Results:

  • The new method provides sensitive detection of total proteins in gels.
  • It exhibits a broad linear dynamic range, enabling better quantification.
  • The staining method demonstrates robust operations and improved reliability compared to traditional silver nitrate staining.
  • The fluorogenic sensing replaces the problematic chromogenic reaction.

Conclusions:

  • A novel fluorescence silver staining method based on a fluorogenic Ag+ probe and AIE has been developed.
  • This method offers significant advantages over traditional silver staining for in-gel protein detection.
  • The new technique provides a sensitive, reliable, and quantifiable alternative for protein visualization in biological research.