Related Experiment Video
Updated: Feb 12, 2026
08:26
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
Published on: October 19, 2015
12.7K
Functionalized TiO2 nanoparticles labelled with 225Ac for targeted alpha radionuclide therapy
Edyta Cędrowska1, Marek Pruszynski1, Agnieszka Majkowska-Pilip1
11Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.
Summary
Titanium dioxide nanoparticles effectively carry Actinium-225 for targeted radionuclide therapy. This novel approach shows promise for treating brain tumors by minimizing toxicity to healthy tissues.
Area of Science:
- Nuclear medicine
- Materials science
- Oncology
Background:
- Actinium-225 (Ac-225) is promising for radionuclide therapy due to its nuclear properties.
- A major challenge is preventing toxic release of radioactive daughters from traditional chelators.
- Titanium dioxide (TiO2) nanoparticles offer a potential solution as carriers for Ac-225 and its decay products.
Purpose of the Study:
- To develop and characterize TiO2 nanoparticles functionalized with Substance P (SP) for targeted delivery of Ac-225.
- To evaluate the stability and retention of Ac-225 and its daughters in the developed bioconjugates.
- To assess the in vitro efficacy of the Ac-225 loaded bioconjugates against glioma cells.
Main Methods:
- Synthesized 25 nm TiO2 nanoparticles functionalized with SP(5-11) peptide via a silan-PEG-NHS linker.
- Characterized nanoparticle-peptide conjugates using transmission electron microscopy (TEM), dynamic light scattering (DLS), and thermogravimetric analysis (TGA).
- Labeled the conjugates with Ac-225 via ion-exchange and evaluated Ac-225 and its daughter (Francium-221) retention in various media, alongside in vitro cytotoxicity assays on T98G glioma cells.
Main Results:
- TiO2-bioconjugate nanoparticles were synthesized with approximately 80 peptide molecules per nanoparticle.
- Ac-225 was retained with high efficiency (>97%) in phosphate-buffered saline (PBS), physiological salt, and cerebrospinal fluid (CSF) for up to 10 days.
- Minimal leaching (30%) of the first daughter, Francium-221 (Fr-221), was observed only in CSF after 10 days.
- The synthesized Ac-225-TiO2-PEG-SP(5-11) demonstrated significant in vitro cytotoxicity against T98G glioma cells.
Conclusions:
- TiO2 nanoparticles provide a stable platform for carrying Ac-225 and managing its radioactive daughters.
- The SP(5-11) functionalization enables targeted delivery to glioma cells.
- This Ac-225-loaded TiO2-bioconjugate represents a promising new radioconjugate for targeted radionuclide therapy of brain tumors.
Related Concept Videos
02:57Targeted Cancer Therapies
9.0K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
9.0K
00:59Gene Therapy
27.7K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.7K
01:26Group Therapy
460
Group therapy is a sociocultural approach to psychological treatment, where individuals with shared psychological challenges come together under the guidance of a mental health professional. This therapeutic modality offers unique opportunities for individuals to connect, share, and grow within the context of a supportive group. By fostering mutual understanding and collaboration, group therapy can address a range of psychological concerns effectively, often complementing or surpassing the...
460
01:22Behavior Therapy
735
Behavior therapy incorporates diverse techniques rooted in classical conditioning principles to address maladaptive behaviors and anxiety disorders. These methods aim to reduce avoidance behaviors, foster adaptive coping mechanisms, and alter associations between stimuli and responses, making them effective in a wide range of therapeutic contexts.
Exposure therapy is a cornerstone of behavioral treatment for anxiety disorders. It involves systematic exposure to feared stimuli, either in real...
Exposure therapy is a cornerstone of behavioral treatment for anxiety disorders. It involves systematic exposure to feared stimuli, either in real...
735
01:28Drug Therapy
304
The advent of drug therapy has profoundly shaped modern mental health care, providing targeted treatments for a range of psychological disorders. Psychotherapeutic drugs, classified into antianxiety, antidepressant, and antipsychotic medications, address symptoms across anxiety disorders, mood disorders, and schizophrenia. While these medications have transformed patient outcomes, they require careful management due to their potential side effects and limitations.
Antianxiety Medications
Antianxiety Medications
304
01:25Cognitive Therapy
1.3K
Cognitive therapy, pioneered by Aaron T. Beck in the 1960s, is a structured approach to addressing psychological distress by focusing on the influence of thoughts on emotions and behaviors. All cognitive therapies involve the basic assumption that human beings have control over their feelings, and that how individuals feel about something depends on how they think about it. Unlike psychoanalytic methods that delve into unconscious processes or humanistic approaches emphasizing...
1.3K