Related Experiment Video

Updated: Feb 12, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K

Downregulated TRPV1 Expression Contributes to Melanoma Growth via the Calcineurin-ATF3-p53 Pathway

Yuqi Yang1, Weinan Guo1, Jingjing Ma1

  • 1Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.

The Journal of Investigative Dermatology
|March 28, 2018
PubMed

Insights

Transient receptor potential vanilloid 1 (TRPV1) acts as a tumor suppressor in melanoma. Lower TRPV1 expression correlates with melanoma, and its activation inhibits cancer growth by inducing apoptosis via p53.

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Melanoma is an aggressive skin cancer with poor prognosis despite recent therapeutic advances.
  • Understanding melanoma pathogenesis is crucial for developing novel treatments.
  • The role of Transient Receptor Potential Vanilloid 1 (TRPV1) in melanoma is currently unknown.

Purpose of the Study:

  • To investigate the role of TRPV1 in melanoma development and progression.
  • To determine if TRPV1 expression is altered in melanoma tissues.
  • To explore TRPV1's potential as a therapeutic target in melanoma.

Main Methods:

  • Quantitative analysis of TRPV1 expression in melanoma tissues and cell lines versus normal controls.
  • In vitro studies involving TRPV1 overexpression and capsaicin treatment in melanoma cells.
  • Investigation of downstream signaling pathways, including p53 activation and apoptosis induction.
  • In vivo studies to confirm the effect of TRPV1 on melanoma growth.

Main Results:

  • TRPV1 expression was significantly downregulated in melanoma tissues and cell lines compared to nevus tissues and normal melanocytes.
  • Overexpression of TRPV1 or treatment with the agonist capsaicin inhibited melanoma cell proliferation and induced apoptosis.
  • TRPV1 activation led to increased intracellular calcium (Ca2+), which in turn activated p53 through the calcineurin-ATF3 pathway.
  • In vivo experiments confirmed that TRPV1 suppresses melanoma tumor growth.

Conclusions:

  • TRPV1 functions as a tumor suppressor in melanoma.
  • Reduced TRPV1 expression is associated with melanoma.
  • TRPV1 activation represents a potential therapeutic strategy for melanoma treatment.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.5K

Related Concept Videos

Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.8K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.3K
Wechsler's Contribution to Measures of Intelligence01:23

Wechsler's Contribution to Measures of Intelligence

David Wechsler, a psychologist who worked with World War I veterans, developed a significant IQ test in 1939 called the Wechsler-Bellevue Intelligence Scale. This test was innovative because it combined several subtests that measured both verbal and nonverbal skills, reflecting Wechsler's belief that intelligence is a global capacity involving purposeful action, rational thinking, and effective interaction with the environment. This test later evolved into the Wechsler Adult Intelligence...
2.1K
Meristems and Plant Growth02:36

Meristems and Plant Growth

Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.7K
Population Growth00:57

Population Growth

Population size is dynamic, increasing with birth rates and immigration, and decreasing with death rates and emigration. In ideal conditions with unlimited resources, populations can increase exponentially, which plots as a J-shaped growth rate curve of population size against time. This type of curve is characteristic of newly-introduced invasive species, or populations that have suffered catastrophic declines and are rebounding.
28.7K