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Related Concept Videos

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
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Gravimetry: Inorganic And Organic Precipitating Agents00:49

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In gravimetry, the precipitant is chosen carefully to obtain a pure solid that can be easily filtered. Common inorganic precipitants can be used to determine several cations and anions. In some cases, the formation of the same precipitate can be used to determine the cation and the anion. For example, the reaction of barium and chromate ions to give barium chromate is used to determine both barium and chromate. However, precipitates such as hydroxides, oxalates, and metal ammonium phosphates...
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The Nitrogen Cycle01:49

The Nitrogen Cycle

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Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
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Overview of Nitrogen Metabolism01:20

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Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
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All living things are formed mostly of carbon compounds called organic compounds. The category of organic compounds includes both natural and synthetic compounds that contain carbon. Although a single, precise definition has yet to be identified by the chemistry community, most agree that a defining trait of organic molecules is the presence of carbon as the principal element, bonded to hydrogen and other carbon atoms. However, some carbon-containing compounds such as carbonates, cyanides, and...
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Chirality at Nitrogen, Phosphorus, and Sulfur02:30

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Chirality is most prevalent in carbon-based tetrahedral compounds, but this important facet of molecular symmetry extends to sp3-hybridized nitrogen, phosphorus and sulfur centers, including trivalent molecules with lone pairs. Here, the lone pair behaves as a functional group in addition to the other three substituents to form an analogous tetrahedral center that can be chiral.
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
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Determining Inorganic and Organic Nitrogen.

Jaana Koistinen1, Mervi Sjöblom2, Kristian Spilling3

  • 1Tvärminne Zoological Station, University of Helsinki, Hanko, Finland. jaana.koistinen@helsinki.fi.

Methods in Molecular Biology (Clifton, N.J.)
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PubMed
Summary

Accurate measurement of algal nitrogen (N) is crucial for cultivation. This study details methods for quantifying dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON) in algae.

Keywords:
AmmoniumDissolved organic nitrogenNitrateNutrient uptakeParticulate organic nitrogen

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Area of Science:

  • * Aquatic microbiology and phycology
  • * Biogeochemical nutrient cycling

Background:

  • * Nitrogen (N) is an essential nutrient for algal growth, forming integral parts of cellular components like proteins.
  • * Precise quantification of nitrogen pools is critical for optimizing algal cultivation and understanding aquatic ecosystems.

Purpose of the Study:

  • * To present established methodologies for determining dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON) in algal samples.
  • * To provide a comprehensive overview of nitrogen analysis techniques relevant to algal research and cultivation.

Main Methods:

  • * Dissolved inorganic nitrogen (DIN) determination utilizes established colorimetric analytical methods.
  • * Dissolved organic nitrogen (DON) and particulate organic nitrogen (PON) are quantified using filtration followed by high-temperature catalytic oxidation.

Main Results:

  • * The study outlines reliable methods for the accurate determination of all major nitrogen fractions in algal biomass and water.
  • * These methods enable detailed assessment of nitrogen availability and utilization in algal systems.

Conclusions:

  • * The presented analytical methods are essential for effective algal cultivation and research.
  • * Accurate nitrogen speciation is key to managing and enhancing algal productivity and ecological roles.