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Area of Science:

  • Biochemistry
  • Cell Biology
  • Chemical Biology

Background:

  • Hydrogen polysulfides (H2Sn, n > 1) are crucial signaling molecules involved in various cellular processes.
  • Accurate detection and localization of H2Sn are essential for understanding their biological roles.
  • Existing methods for H2Sn detection may have limitations in terms of specificity, cell permeability, or imaging capabilities.

Purpose of the Study:

  • To develop and characterize a novel molecular probe for the specific detection of intracellular hydrogen polysulfides.
  • To investigate the intracellular localization and distribution of H2Sn within cells.
  • To establish a reliable tool for visualizing H2Sn dynamics using advanced microscopy.

Main Methods:

  • Synthesis and characterization of a new BODIPY-based molecular probe, MB-Sn.
  • Evaluation of the probe's physiological benignity and cell membrane permeability.
  • Application of MB-Sn for sensing intracellular H2Sn in the endoplasmic reticulum.
  • Imaging of H2Sn distribution using wide-field and super-resolution Structured Illumination Microscopy (SIM).

Main Results:

  • MB-Sn was found to be physiologically benign and permeable across cell membranes.
  • The probe specifically sensed intracellular hydrogen polysulfides (H2Sn, n > 1).
  • MB-Sn successfully localized H2Sn within the endoplasmic reticulum.
  • The probe enabled effective mapping of H2Sn distribution via wide-field and SIM imaging.

Conclusions:

  • MB-Sn is a valuable and specific molecular probe for detecting intracellular H2Sn.
  • The probe's properties make it suitable for visualizing H2Sn in the endoplasmic reticulum.
  • MB-Sn facilitates the study of H2Sn dynamics and distribution using advanced microscopy techniques.