Increased diene conjugates in diabetic subjects with microangiopathy

Free radicals are highly reactive unstable chemical species which have been implicated in the vascular damage associated with several disease states. Diene conjugates, probable products of free radical activity on lipids, were measured spectrophotometrically in 26 diabetic patients with microangiopathy, 36 uncomplicated diabetic patients, and 36 healthy controls. Total diene conjugates and diene conjugate to triglyceride ratios were significantly elevated in diabetic patients with microangiopathy (0.57 +/- 0.08 and 0.31 +/- 0.14 OD units/ml respectively; mean +/- SD) when compared with patients without complications (0.32 +/- 0.10, p less than 0.001, and 0.17 +/- 0.06, OD units/ml, p less than 0.011). There was no difference in total diene conjugation and their ratio to triglycerides between healthy controls and uncomplicated diabetic patients and the results were not influenced by the type of diabetes or level of diabetic control. Diene conjugate ratios repeated in 14 patients on two occasions, 6 weeks apart, with stable metabolic control were consistent. Increased diene conjugation suggesting increased free radical activity is associated with microangiopathy and this may have pathogenetic implications.