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Published on: October 11, 2016
Voltammetric determination of 5-hydroxytryptamine based on the use of platinum nanoparticles coated with molecularly
Yiwen Yang1, Yanbo Zeng2, Chuangui Tang1,3
1College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, People's Republic of China.
Abstract:
The authors describe an electrochemical sensor for the determination of 5-hydroxytryptamine (5-HT; serotonin). It is based on the use of platinum nanoparticles (PtNPs) coated with molecularly imprinted silica (MIS). The MIS-coated PtNPs were prepared from 5-HT, PtNPs, phenyltrimethoxysilane and tetraethoxysilane that act as template, support, functional monomer and cross-linker, respectively. The MIS-coated PtNPs were characterized by Fourier transfer infrared spectroscopy and transmission electron microscopy. The MIS-coated PtNPs were drop-cast onto a glassy carbon electrode (GCE) to obtain an electrochemical sensor for 5-HT which exhibited fast response and high recognition ability for 5-HT. The imprinting factor of this electrode for 5-HT is 4.12, which is higher than that for its analogs. Under optimized conditions and at a typical working potential of 0.29 V (vs. Hg/Hg2Cl2), the electrode has a linear response in the 0.05-80 μM 5-HT concentration range and a 0.02 μM detection limit. The electrode was successfully applied to the determination of 5-HT in human serum samples. Graphical Abstract Molecularly imprinted silica-coated platinum nanoparticles were prepared using surface imprinting technique. A glassy carbon electrode modified with the surface imprinted material exhibits fast response in detecting 5-hydroxytryptamine, a low detection limit, good selectivity, reproducibility and stability.
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