Fluorescence from a single Symbiodinium cell
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Updated: Feb 12, 2026

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Published on: August 18, 2018
Christine Guzman1, Xue Han, Eiichi Shoguchi
1Light-Matter Interactions Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan. Evolutionary Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
Abstract:
The partnership between coral and its algal symbionts, Symbiodinium, is crucial to the global environment. Yet, the regulatory process within the photosynthetic machinery of Symbiodinium is still not clearly understood. Here, we studied the influence of light stress from focussed red and blue lasers on single Symbiodinium cells. Fluorescence signals were measured to show cell response. Increasing the incident laser power or the exposure time resulted in an increase followed by a decline in fluorescence intensity. The trend of fluorescence intensity changes was associated with mechanisms of light use efficiency, non-photochemical quenching, photoinhibition, and repair of the cell. Our study provides new approaches to studying the photobiology and physiology of Symbiodinium cells.
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