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10:46
In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
Published on: March 16, 2018
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Nanomagnetic Modulation of Tumor Redox State
Valerii E Orel1, Marina Tselepi2, Thanos Mitrelias3
1Medical Physics and Bioengineering Research Laboratory, National Cancer Institute, Kyiv, Ukraine; Biomedical Engineering Department, NTUU "Igor Sikorsky KPI", Kyiv, Ukraine.
Nanomedicine : Nanotechnology, Biology, and Medicine
|March 30, 2018
Summary
Magnetic nanodots loaded with doxorubicin show enhanced antitumor effects in carcinosarcoma by modulating tumor redox state. This novel nanotherapy approach offers improved efficacy compared to conventional doxorubicin treatment.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Oncology
Background:
- Tumor microenvironment modulation via reactive oxygen and nitrogen species presents a therapeutic target.
- Doxorubicin-loaded iron oxide nanoparticles offer potential for cancer nanotherapy.
Purpose of the Study:
- To investigate the antitumor efficacy of doxorubicin-loaded magnetic nanodots combined with electromagnetic fields in Walker-256 carcinosarcoma.
- To evaluate the modulation of tumor redox state by these magnetic nanodots.
Main Methods:
- Synthesis of doxorubicin-loaded Fe3O4 magnetic nanodots.
- In vivo treatment of Walker-256 carcinosarcoma in rats.
- Assessment of tumor growth inhibition and redox state using electron spin resonance (ESR) spectroscopy.
Main Results:
- Magnetic nanodots with the largest hysteresis loop area demonstrated superior antitumor effect, reducing tumor growth factor to 0.49 day⁻¹ compared to 0.58 day⁻¹ for conventional doxorubicin.
- ESR spectra revealed a 2.7-fold increase in free iron, promoting reactive oxygen species, and increased ubisemiquinone, lactoferrin, and NO-FeS-proteins in tumors treated with optimized nanodots.
Conclusions:
- Designed magnetic nanodots effectively modulate the tumor redox state, enhancing antitumor activity.
- This nanotherapeutic strategy shows promise for improved cancer treatment by targeting tumor redox balance.
![Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F68356.jpg&w=3840&q=50)
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