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Molecular genetic analysis of the pyr-4 gene of Neurospora crassa
J A Glazebrook1, K Mitchell, A Radford
1Department of Genetics, University of Leeds, UK.
Molecular & General Genetics : MGG
|September 1, 1987
Abstract:
By means of S1 nuclease mapping, one transcription origin and three termini are identified for the pyr-4 gene of Neurospora crassa, the same origin being used also by Escherichia coli on the cloned gene. Translation of the clone in mini-cells gives a 50,000 dalton gene product, the same size as that determined for the Neurospora native enzyme. Putative CAAT and TATA boxes, and upstream and downstream potential secondary structures, are identified.