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Show Yourself, Asparaginase: An Enzymatic Reaction Explained through a Hands-On Interactive Activity
Josell Ramirez-Paz1, Bonny M Ortiz-Andrade2,3, Kai Griebenow1
1Department of Chemistry, Faculty of Natural Sciences, University of Puerto Rico, Rio Piedras Campus, P.O. Box 70377, San Juan, Puerto Rico 00936-8377, United States.
Journal of Chemical Education
|March 31, 2018
Summary
This study introduces an inexpensive, hands-on classroom activity to identify enzymes like asparaginase by measuring their catalytic activity. It aims to inspire students about protein-based drugs and scientific careers.
Area of Science:
- Biochemistry
- Biotechnology Education
Background:
- Enzyme identification is crucial for purification and isolation.
- Protein-based drugs represent an emerging field with career potential.
Purpose of the Study:
- To develop an accessible, interactive classroom activity demonstrating enzymatic activity.
- To distinguish a medically relevant enzyme (asparaginase) from a nonenzymatic protein.
- To promote bionanotechnology careers and a positive image of scientists.
Main Methods:
- Designed a hands-on experiment for classroom use.
- Utilized asparaginase as the model enzyme.
- Adapted the activity for diverse educational levels (elementary to high school).
Main Results:
- Successfully distinguished asparaginase from a nonenzymatic protein based on catalytic activity.
- The activity is inexpensive and adaptable to various educational settings.
- Demonstrated the practical application of enzyme kinetics in a learning environment.
Conclusions:
- Enzymatic activity is a viable method for enzyme identification in educational settings.
- This activity effectively engages students with protein-based drug concepts and bionanotechnology.
- The experiment provides a realistic and accessible portrayal of scientific work.
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