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Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion
Published on: July 6, 2022
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Proteomic Protocol in Esophageal Adenocarcinoma
1Axis Shield, Dundee, Scotland, UK. peter.kelly@alere.com.
Methods in Molecular Biology (Clifton, N.J.)
|March 31, 2018
Summary
Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) can reveal esophageal adenocarcinoma proteomic profiles. These profiles may predict survival and monitor chemotherapy response in patients.
Area of Science:
- Biochemistry
- Proteomics
- Oncology
Background:
- Esophageal adenocarcinoma is a significant cause of cancer mortality.
- Accurate prognostic and predictive biomarkers are needed for patient management.
- Proteomic profiling offers a potential avenue for biomarker discovery.
Purpose of the Study:
- To present a standardized protocol for proteomic profiling in esophageal adenocarcinoma.
- To establish serum or plasma protein profiles using SELDI-TOF mass spectrometry.
- To facilitate the identification of potential biomarkers for survival prediction and chemotherapy response monitoring.
Main Methods:
- Sample preparation from esophageal adenocarcinoma patient serum or plasma.
- Analysis of proteomic profiles utilizing SELDI-TOF mass spectrometry.
- Collection and identification of protein fractions corresponding to significant peaks.
Main Results:
- Demonstration of a reproducible protocol for SELDI-TOF MS analysis in esophageal adenocarcinoma.
- Establishment of distinct proteomic profiles in patient samples.
- Foundation laid for identifying specific protein biomarkers.
Conclusions:
- SELDI-TOF mass spectrometry is a viable technique for esophageal adenocarcinoma proteomic profiling.
- Proteomic profiles hold promise for predicting patient survival.
- These profiles may serve as indicators for monitoring chemotherapy response.
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