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Updated: Feb 12, 2026

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Published on: August 13, 2010
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique
Sébastien Aimé1,2, Siham Hichami1,2, David Wendehenne1,2
1UMR 1347 Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne-Franche Comté, Dijon Cedex, France.
Abstract:
Nitric oxide is regarded as a key signaling messenger in several organisms. Its physiological relevance is partly due to its capacity to induce posttranslational modifications of proteins through its direct or indirect reaction with specific amino acid residues. Among them, S-nitrosylation has been shown to be involved in a broad range of cellular signaling pathways both in animals and plants. The identification of S-nitrosylated proteins has been made possible by the development of the Biotin-Switch Technique (BST) in the early 2000s. Here, we describe the BST protocol we routinely use to check in vitro S-nitrosylation of recombinant proteins induced by NO donors.
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