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Synthetic Antigen Controls for Immunohistochemistry
Published on: August 23, 2021
Precision in Diagnostic Molecular Pathology based on Immunohistochemistry
Ru Fang1, Xiao-Tong Wang2, Qiu-Yuan Xia1
1Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, China.
Immunohistochemistry (IHC) provides a cost-effective and efficient method for molecular pathological diagnosis. This review covers IHC biomarkers for assessing tumor genetics and molecular changes, noting their limitations.
Area of Science:
- Pathology
- Oncology
- Biochemistry
Background:
- Immunohistochemistry (IHC) is crucial for molecular pathological diagnosis.
- It aids in differentiating tumors, determining origin, assessing cell differentiation, and detecting microorganisms.
- IHC enables protein detection for evaluating tumor gene information and molecular pathological changes.
Purpose of the Study:
- To review IHC biomarkers used in molecular pathology.
- To highlight IHC's role in reflecting or replacing molecular pathology assessments.
- To discuss the limitations and precautions for IHC applications.
Main Methods:
- Literature review of immunohistochemistry applications in pathology.
- Analysis of IHC biomarkers for molecular pathology.
- Evaluation of IHC's advantages over other molecular detection tools.
Main Results:
- IHC is a cost-effective, time-efficient, and labor-saving diagnostic tool.
- Specific IHC biomarkers can effectively reflect or substitute for molecular pathology analyses.
- Identified shortcomings and necessary precautions for IHC use.
Conclusions:
- Immunohistochemistry is an indispensable and efficient tool in routine pathological diagnosis.
- IHC biomarkers offer valuable insights into molecular pathology and tumor characteristics.
- Awareness of IHC limitations is essential for accurate diagnostic applications.
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