Prenylflavonoids from the Twigs of Artocarpus nigrifolius

Two new prenylated flavones, artocarnin A (2) and carpachromenol (12), together with 13 known prenylflavonoids (1, 3-11, 13-15) were isolated from the twigs of Artocarpus nigrifolius for the first time. Their structures were elucidated by high resolution-electrospray ionization (HR-ESI)-MS, NMR spectroscopic analysis, and in comparison with the reported data. Compounds 1-15 were evaluated for their antiproliferative effects against SiHa and SGC-7901 human cancer cell lines in vitro. The most active compound, eleocharin A (10), showed significant cytotoxicity on SiHa cells (IC₅₀=0.7±0.1 µM) and inhibitory activity against SGC-7901 cells (IC₅₀=8.3±0.2 µM) and could be considered as potential lead compound for further development of novel anti-tumor agents.