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Related Concept Videos

Glassware Calibration01:11

Glassware Calibration

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Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Instrument Calibration01:12

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Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Human behavior is intricately shaped by social influences that arise from interactions with others in diverse contexts. These influences not only mold beliefs and attitudes but also drive the regulation of behaviors through both direct communication and observational learning. The study of these processes falls within the domain of social psychology, which seeks to understand how individuals are affected by and affect those around them.Mechanisms of Social InfluenceDirect social influence...
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Calibration Curves: Correlation Coefficient01:10

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In a linear calibration curve, there is a value called the calibration coefficient, denoted by 'r,' which measures the strength and the direction of association between two variables. The correlation coefficient value ranges from −1 to +1. A value of +1 indicates a perfect positive linear correlation, −1 denotes a perfect negative correlation, and 0 implies no correlation between the two variables. A positive correlation value establishes that as one variable increases, the...
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Related Experiment Video

Updated: Feb 12, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
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How To Characterize Individual Nanosize Liposomes with Simple Self-Calibrating Fluorescence Microscopy.

Kim I Mortensen1, Chiara Tassone1, Nicky Ehrlich1

  • 1Department of Micro- and Nanotechnology , Technical University of Denmark , Kongens Lyngby , DK-2800 , Denmark.

Nano Letters
|April 4, 2018
PubMed
Summary

Characterizing nanosize lipid vesicles is crucial for nanotechnology and biology applications. This study introduces a dual-color fluorescence method to individually assess vesicle properties, revealing that only a fraction meet ideal structural and encapsulation standards.

Keywords:
Liposomesdual-color fluorescence microscopyencapsulation efficiencylamellaritysingle-particle analysisvesicles

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Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
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Area of Science:

  • Nanotechnology and biology interface
  • Lipid vesicle characterization
  • Biophysical analysis

Background:

  • Nanosize lipid vesicles are vital for drug delivery and chemical reactions.
  • Vesicle samples typically exhibit heterogeneity in size and structure.
  • Individual vesicle characterization is essential for accurate experimental interpretation.

Purpose of the Study:

  • To develop a method for individual characterization of nanosize lipid vesicles.
  • To identify ideal vesicles based on size, shape, lamellarity, and encapsulation efficiency.
  • To enable accurate calibration of dual-color fluorescence microscopy images.

Main Methods:

  • Dual-color fluorescence labeling of lipid bilayers and lumens.
  • Image analysis of vesicle spots to determine intensity and width.
  • Calibration of microscopy images using ideal vesicles identified in situ.

Main Results:

  • A method was established to yield size, shape, lamellarity, and encapsulation efficiency for each vesicle.
  • Ideal vesicles were identified based on specific imaging data relationships.
  • Non-ideal vesicles were characterized by deviations from the calibrated relationships.

Conclusions:

  • The developed method allows for precise characterization of individual nanosize lipid vesicles.
  • Contrary to assumptions, only a fraction of extruded vesicles are structurally ideal.
  • In situ calibration using ideal vesicles enhances microscopy accuracy and reduces error.