Related Experiment Video
Updated: Feb 12, 2026
A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft
Published on: August 8, 2020
Candida parapsilosis isolates from burn wounds can penetrate an acellular dermal matrix
Erika Okuno1, Isabele Carrilho Jarros2, Patricia Souza Bonfim-Mendonça2
1Laboratory of Medical Mycology, State University of Maringá, Brazil; Burn Treatment Center of the North Paraná University Hospital, State University of Londrina, Brazil.
Abstract:
We isolated and identified yeasts from burn wounds and evaluated the ability of Candida parapsilosis isolates from burn wounds to penetrate an acellular dermal matrix (ADM). A prospective study was conducted with patients from the burn treatment center of North Paraná University Hospital in Londrina, Brazil from February 2015 to January 2016. Yeast cultures were obtained from the tissue of burn wounds that had been debrided and cleansed with 2% chlorhexidine. After identification and confirmation of the purity of the culture, the yeasts were placed on ADM fragments and incubated for three or seven days. During the study period, 273 patients were treated, and 36 of these patients fulfilled the inclusion criteria and provided samples for culture. Yeasts were isolated in 19.44% (n = 7) of the cultures, and the following species were identified: C. parapsilosis (57.1%), C. albicans (28.6%), and C. glabrata (14.3%). C. parapsilosis, the most frequent species, was chosen for the ADM tests. We demonstrated active penetration of the ADM by the yeast isolates from burn wounds. C. parapsilosis grew on ADM and penetrated the matrix, indicating that this yeast, which is common in skin and cutaneous wounds, has the potential to colonize and pass through ADM, a medical device that is frequently used to dress and regenerate burn wounds.
Insights
Candida parapsilosis yeasts were isolated from burn wounds and shown to actively penetrate acellular dermal matrix (ADM). This finding highlights the potential for yeast colonization and matrix traversal in burn wound treatment.
Area of Science:
- Medical Mycology
- Wound Healing Research
- Biomaterials Science
Background:
- Burn wounds are susceptible to microbial infections, complicating healing.
- Acellular dermal matrix (ADM) is a common biomaterial used in burn wound management.
- The potential for microorganisms to breach ADM requires investigation.
Purpose of the Study:
- To isolate and identify yeasts from burn wound infections.
- To evaluate the in vitro capacity of Candida parapsilosis to penetrate ADM.
- To assess the clinical relevance of yeast presence in burn wounds treated with ADM.
Main Methods:
- Prospective study of burn patients from February 2015 to January 2016.
- Yeast cultures obtained from debrided burn wound tissue.
- In vitro incubation of Candida parapsilosis isolates on ADM fragments for 3 and 7 days.
Main Results:
- Yeasts were isolated from 19.44% of burn wound cultures.
- Candida parapsilosis was the most frequent species (57.1%), followed by C. albicans and C. glabrata.
- Candida parapsilosis demonstrated active penetration and colonization of ADM in vitro.
Conclusions:
- Candida parapsilosis, prevalent in burn wounds, can actively penetrate and colonize ADM.
- This yeast's ability to breach ADM poses a potential challenge for burn wound regeneration.
- Further research is needed to understand and mitigate yeast penetration of ADM in clinical settings.
Related Concept Videos
01:22Burn Injuries
The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...
01:42The Extracellular Matrix
01:29The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:26Extracellular Matrix
01:18The Bone Matrix
01:28Phases of Wound Repair
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...