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A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
Published on: May 26, 2022
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A Key Gene, PLIN1, Can Affect Porcine Intramuscular Fat Content Based on Transcriptome Analysis
Bojiang Li1, Qiannan Weng2, Chao Dong3
1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, 210095 Nanjing, China. 2015205004@njau.edu.cn.
Genes
|April 5, 2018
Summary
This study identifies perilipin-1 (PLIN1) as a key gene in intramuscular fat deposition in pigs. PLIN1 promotes fat accumulation and adipocyte differentiation, offering insights into meat quality.
Area of Science:
- Animal Science
- Molecular Biology
- Genetics
Background:
- Intramuscular fat (IMF) content is crucial for meat quality.
- The genetic and molecular mechanisms regulating IMF deposition are not fully understood.
Purpose of the Study:
- To identify key genes involved in porcine IMF deposition.
- To investigate the role of perilipin-1 (PLIN1) in IMF accumulation and adipocyte differentiation.
Main Methods:
- Transcriptome analysis to identify differentially expressed genes (DEGs) between high and low IMF pigs.
- Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
- Quantitative real-time PCR (qRT-PCR) to analyze PLIN1 expression.
- In vitro knockdown experiments in porcine adipocytes.
Main Results:
- 75 DEGs were identified, with PLIN1 significantly enriched in the peroxisome proliferator-activated receptor (PPAR) signaling pathway.
- Porcine PLIN1 showed high expression in adipose tissue, increased in high IMF groups and during adipocyte differentiation.
- PLIN1 knockdown reduced triglyceride levels and lipid droplet size in porcine adipocytes.
Conclusions:
- PLIN1 is a novel candidate gene significantly associated with porcine IMF content.
- PLIN1 plays a critical role in promoting IMF deposition and adipocyte differentiation in pigs.
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