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Published on: November 21, 2013
Tunable Ampere phase plate for low dose imaging of biomolecular complexes
Amir H Tavabi1, Marco Beleggia2, Vadim Migunov3
1Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich, 52428, Jülich, Germany. a.tavabi@fz-juelich.de.
Abstract:
A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.
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